Continous Real-Time Cell Analysis - Highly sensitive, non-invasive, easy to operate 

Immune Cell Killing Analysis

Enhance cancer immunotherapy research with xCELLigence Real-Time Cell Analysis. This label-free, in vitro assay provides sensitive, accurate, and direct measurements of immune cell killing potency. Monitor cellular mechanisms, including serial killing and exhaustion, under physiological conditions.

Cytotoxicity Assays`

Assess the cytotoxic effects of drugs or compounds by monitoring cell proliferation, size, morphology, and attachment quality in real time. Monitor the kinetics of long-term cellular responses with this simple and high-throughput cytotoxicity assay. Effortlessly generate dose-response curves at multiple time points, make informed decisions about treatment timing, and all without increasing workload.

Cell Adhesion Assays

Circulating tumor cells (CTCs) play a key role in metastasis by reattaching, extravasating, and spreading to distant organs. With xCELLigence RTCA, continuously track cell adhesion kinetics in real time—without fluorescent dyes. Gain deeper insights into metastatic processes and quantitatively assess cell attachment and spreading with a label-free, automated workflow.

Apoptosis and Proliferation Assays

Discover simple, quantitative, high-throughput apoptosis and proliferation assays that offer multiple perspectives on cell health and viability. Monitor real-time cell health, capturing early and late apoptotic events such as changes in cell adhesion, membrane integrity, and detachment. Track host cell proliferation to assess drug toxicity or viral presence. Detect and quantify apoptosis in living cells using a label-free approach or apoptotic/nuclear labeling reagents. The xCELLigence RTCA eSight delivers comprehensive insights with impedance, fluorescence, and brightfield imaging, providing multiple temporal vantage points.

Cell Invasion and Migration Assays

Cell invasion and migration are essential in processes like wound healing and cancer metastasis. Traditional methods often lack quantitative accuracy and efficiency. The xCELLigence RTCA DP system provides a solution by automatically collecting migration data over extended periods—without the need for sample processing, staining, or manual counting. The RTCA software generates kinetic migration curves automatically, offering a reproducible and efficient approach to studying cell movement in real time.

Stem Cell Assays

Stem cell research provides mechanistic insights into disease, aids regenerative medicine development, and offers an alternative approach to test the liability and effectiveness of new drugs. Overcome cell therapy manufacturing challenges by predicting functional capacity and assessing variability of mesenchymal stem cells (MSCs) with xCELLigence RTCA. Maximize production yields using fewer cells and continuously measure integrated changes in cell number, attachment, and morphology to ensure consistency between passages.

Cell Barrier Function Assays

Endothelial and epithelial cell barriers play a crucial role in protecting against disease but can become compromised in various conditions. xCELLigence RTCA provides a sensitive, impedance-based alternative to traditional barrier function assays like solute permeability and TEER. Continuously monitor barrier integrity and disruption in real time with a label-free, quantitative approach for superior insights into disease mechanisms.

Cell Signaling Assays

GPCR, RTK, and nuclear hormone signaling drive biochemical changes that impact cell count, morphology, and adhesion. xCELLigence RTCA enables real-time, label-free tracking of cell size, shape, and proliferation in response to signaling events. Capture dynamic cellular responses within minutes and monitor changes over days with a continuous, quantitative workflow.

Viral Cytopathic Effect Assays

Optimize your antiviral research by measuring viral cytopathic effects (CPE) in real time—without labor-intensive plaque assays. xCELLigence RTCA instruments automate CPE monitoring from minutes to days, reducing workload and manual handling. Effortlessly track cell proliferation kinetics, determine the ideal infection time based on cell seeding density, and assess virus-mediated cytopathogenicity—all in a single, streamlined workflow.

Phagocytosis Assays

Dynamically analyze key immune mechanisms involved in pathogen clearance and cell debris removal. Track the entire process in real time through imaging, screen for pathway modulators, and identify potential therapeutic interventions. Visualize and label macrophages using nuclear labeling reagents for enhanced insights into immune responses.

The CiPA (Comprehensive In Vitro Proarrhythmia Assay) Initiative

Driven by HESI, FDA, and the Safety Pharmacology Society, the CiPA initiative aims to enhance cardiac safety assessment by evaluating potential modifications to current FDA guidelines. The xCELLigence RTCA CardioECR was selected as a core technology for validation, playing a role in both Phase I and II of this initiative.

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Cardiac Disease Modeling

Model pathophysiological cellular phenotypes of genetically heritable heart diseases using disease-specific human induced pluripotent stem cell cardiomyocytes (hiPSC-CM). This modeling approach offers deeper insights into the mechanisms behind compromised electrical and contractile coupling in heart disease.

Cardio Safety Toxicology

Human induced pluripotent stem cell cardiomyocytes (hiPSC-CM) are ideal for assessing drug liability. The xCELLigence RTCA CardioECR system provides a unique multiplex detection method to probe and understand the underlying toxicity of compounds and pharmaceuticals, enabling more accurate and reliable results.

Cardio Drug Discovery

Human induced pluripotent stem cell cardiomyocytes (hiPSC-CM) provide an advanced model for evaluating the efficacy and toxicity of new therapeutic approaches. This includes testing inotropic compounds that modulate cardiomyocyte contractility, offering valuable insights into their potential effects on heart function.

Cardio Maturation

Long-term electrical pacing is becoming the preferred method to improve the maturation of hiPSC-CMs in a consistent, scalable way. This approach enhances gene and protein expression, as well as contractile response, making it ideal for drug safety testing, cardiovascular drug discovery, and disease modeling.