Real-Time Analysis with Impedance Monitoring and Live-Cell Imaging
xCELLigence RTCA eSight - Real-Time Insights into Cell Health, Behavior, and Function.
xCELLigence RTCA eSight - currently the only platform on the market that combines impedance monitoring with multi-color live-cell imaging at the same time
Your Challenges using Traditional Methods
Only end-point data available.
Invasive & destructive techniques.
Low sensitivity and delayed detection.
Poor reproducibility and high variability.
Lack of multiparametric insights.
Time-consuming and labor-intensive.
xCELLigence RTCA eSight delivers the solution
Continuous Real-Time Monitoring
Label-Free Impedance Technology combined with Live-Cell Imaging
Increased Reproducibility & Efficiency
Higher Sensitivity for Early Detection
Intuitive Data Analysis RTCA Software
xCELLigence RTCA eSight: Simplify your workflow and gain
physiologically relevant real-time data with ease.
Advanced Live-Cell Analysis with xCELLigence eSight
Impedance Monitoring & Live-Cell Imaging at the Same Time
Integrated Impedance
& Imaging
Combine label-free real-time impedance analysis with high-resolution live-cell imaging in
red, green, and blue, utilizing 5x, 10x, and 20x objectives for enhanced
confidence in your results.
Whole-Well Imaging
Expand your view with whole-well imaging, enabling thorough QC, precise cell growth monitoring, and accurate quantification.
Multiplexed Assays
The power of multiplexing offers information “richness”. The eSight allows the tracking of cell health and behavior simultaneously, and of the same cell populations, from the very different vantage points of real-time cellular impedance and live cell imaging.
Very Fast
Read a 96-well plate in 15 seconds with the xCELLigence impedance technology, and image an entire 96-well plate in only 6 minutes.
Flexible Imaging
Utilize brightfield and three fluorescence channels across various microplate formats (6- to 384-well) and vessel types (35- or 60-mm dishes), with user-defined imaging schedules for maximum flexibility.
Physiologically Relevant Data
in 2D & 3D Cultures
Easily monitor cell health, behavior, and function with a simple workflow in primary or native cells alone or in co-culture, providing unprecedented insight into cellular mechanisms and functionality.
Applications
Immune Cell Killing
Viral Cytopathic Effects
Cytotoxicity
Apoptosis & Proliferation
Phagocytosis
Innovative Software Modules for Live-Cell Analysis
3D Spheroid Invasion
Study invasive potential of tumor spheroids over time as you measure and quantify metastatic potential by label or label-free analysis. Investigate response to treatments and the inhibitory effect on invasion and progression.
Dilution cloning
Easily track cell monoclonal stable cell lines over time using a limited dilution cloning method on eSight. Automatically screen clonal cell lines with minimal effort and high efficiency.
Wound Healing Migration and Invasion
Assess the effects of treatments on cell migration profiles and investigate the rate of wound closure. Explore metastatic potential and how various conditions affect or delay healing. Metrics such as wound width, confluence, cell index, object count, and density are easily measurable with automated software.
Cell-by-cell analysis
Continuously monitor and quantify single cell segmentation by label-free analysis. Gather cell count and nuclear fluorescent recognition count as a function over time. Measure cell subpopulations over time and gain insights into cellular heterogeneity.
Immunotherapy
Now easily calculate and convert to % cytolysis using imaging parameters to expand your Immunotherapy and Immune Cell Killing Analysis and measure potency with ease.
